Until 2001, Anna Mouglalis studied at the Conservatoire National Supérieur d'Art Dramatique de Paris (CNSAD) under the direction of Daniel Mesguich. In 1997, she had begun an acting career in La Nuit du Titanic. In the same year she was chosen by Francis Girod for the film Terminal. In 2000, she co-starred with Isabelle Huppert in Claude Chabrol's Merci pour le chocolat. After appearing in Novo (2002) by Jean - Pierre Limosin, she was cast by Roberto Andò for the thriller Sotto falso nome. In 2003, she played in La Maladie de la mort, a film in black and white by first-time director Asa Mader, which previewed at the Venice FF. In this same year, she co-starred in a Greek film, called Real Life (Alithini Zoi), directed by Panos Koutras. In 2005, she took part in two Italian movies: Romanzo criminale, directed by Michele Placido, and Mare buio, where she was featured alongside Luigi Lo Cascio. In a TV movie called Les Amants du Flore and directed by Ilan Duran Cohen, Mouglalis played Simone de Beauvoir, with Jean-Paul Sartre played by co-star Lorànt Deutsch. Together they had a great public and critical triumph. In 2002, she was chosen by Karl Lagerfeld for the ad campaign for the Amateur Allure de Chanel perfume. He uses her as one of his "muses," promoting Chanel bags, fine jewelry, and watches. Mouglalis was cast as Coco Chanel in the 2009 film Coco Chanel & Igor Stravinsky, directed by Jan Kounen. The film was chosen to close the 2009 Cannes FF.